Molecular cloning of seven truncated cDNAs for human blood coagulation factors with serine protease activity
(0) Coagulation, a biological process highly conserved in evolution with the purpose to form clots, consists of the sophisticated cascade which needs a variety of participants. Disruption of the process can lead to severe consequences, among which are the well-known hemophilia A, B and C, as a result of deficiency of FVIII, FIX and FXI, respectively. To date, a variety of excellent research have been performed on the system, however, it seems necessary to carry out some additional research for some theoretical, practical, clinical or therapeutic attempts. Herein, we separated total RNA from human liver and obtained seven partial cDNA sequences corresponding to FXIIa, FXIa, FXa, FIXa, FVIIa, Flla and plasmin, through RT-PCR according to the submitted sequences of NCBI. Each cDNA sequence has been integrated to pPIC9K in order to express the individual protein and analyze/modify some properties.
coagulation FXIIa FXIa FXa FIXa FVIIa FIIa plasmin
Jiang He-Chun Hua Zi-Chun
State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, China
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
116-119
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）