Preliminary investigation on the correlation between circulating tumor cells and cancer stem cells in patients with breast cancer
In this study, we used anti-epithelial cell adhesion molecule binding to the epithelial cells of peripheral blood mononuclear cells (PBMC) in breast cancer patients, selected the epithelial cells by magnetic-activated cell separation (MACS), then analyzed the enriched epithelial cells labeled with anti-CK-FITC, anti-CD44-PE and anti-CD24-PE-cy5 respectively by FCM. We used the standard that the CK+ cells were testified as circulating tumor cells (CTC) and the CK+ CD44+ CD24-/low cells were attributed to CTC-cancer stem cells (CTC-CSC) in breast cancer patients respectively. The results indicated that the CK+ tumor cells were detected in 19 of 26 patients and the CTC-CSC were identified in 18 of the 19 patients with CTC (94.74％), and the percentage of CTC-CSC in CTC was 19.01％. The results also suggested that the breast cancer patients with the high-rate CK+ tumor cells were in the advanced TNM stage III and the patients with the low-rate CK+ cells were in the modest TNM stage I respectively. The difference between the two groups was statistically significant (p< 0.001). We concluded that there was a significant correlation between CTC and CTC-CSC in breast cancer metastasis.
breast cancer CTC CSC PBMC MACS anti-epithelial cell adhesion molecule
Wang Jing Cao Ming-Gang You Cheng-Zhong Wang Cai-Lian Liu Shen-Li Zhao Feng-Zhu Dou Jun
Department of Gynecology and Obstetrics, Zhongda Hospital, Southeast University, 210009, China Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Southeast Universi Department of Surgery, Zhongda Hospital, Southeast University, Nanjing, 210009, China
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
126-129
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）