Proteomic analysis of mechanical stressed condylar chondrocytes
Mechanical stress is one of the key factors affecting remodeling of condylar cartilage, in which chondrocytes play an important role. However, little knowledge currently exists about this molecular event. To evaluate this mechanism, we developed animal model and cultured stressed chondrocytes, and then took a proteomic approach. Using 2-DE, the gels displayed 1 357±36 protein spots in the control group and 1 295 ± 62 protein spots in the experimental group (IE 3 ～ 10, 14 ～ 195 kD) respectively. Image analysis revealed that mechanical stress significantly modified the expression of proteins, 26 spots down-regulation and 12 spots up-regulation, of which 9 were identified by mass spectrometry (MALDI-TOF).
condylar chondrocytes mechanical stress 2D-gel electrophoresis
Li Huang Wu Tuo-Jiang Wang Wen-Mei Sun Wei-Bin Zhang Xiang-Yu Li Jie Ma Qiao-Ling Hu Qin-Gang Hua Zi-Chun
The Stomatological Hospital of Nanjing University, Nanjing, 210009, China The Stomatological College of Nanjing Medical University, Nanjing, 210028, China The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, China
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
143-145
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）