The F protein of Hepatitis C virus subtype 1b inhibits human hepatocellular carcinom HepG2 cell apoptosis in vitro
To investigate the effects of the F protein of Hepatitis C virus subtype lb on the apoptosis of human hepatocellular carcinom HepG2 cells. HepG2 cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP. And pcDNA3.0-F-EGFP-HepG2 strain was exposed to Act-D and tumor necrosis factor α (TNFα) treatment to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-HepG2, negative control pcDNA3.0-C-EGFP-HepG2and blank control HepG2. Annexin V-FITC/PI of Flow cytometry was performed to determine the number of apoptotic cells. DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotic cells. pcDNA3.0-F-EGFP-HepG2cell strain showed a much delayed apoptosis as well as obviously lowered apoptotic rate in comparison with the pcDNA3.0-HepG2 strain and HepG2 strain (P<0.01). The introduction and expression of extraneous gene (the F gene of Hepatitis C virus subtype 1b) significantly inhibits the apoptosis of HepG2 cells.
the F protein HepG2 apoptosis tumor necrosis factora flow cytometry DNA Ladder
Yang Jing-Jing Zhang Yun Deng Xiao-Zhao Wang Zhong-Can Wang Jie Wang Hao-Peng
Institute of Militalry Medicine, Nanjing Jinling Hospital, Medical School, Nanjing University, Nanji Institute of Militalry Medicine, Nanjing Jinling Hospital, Medical School, Nanjing University, Nanji Department of Epidemiology, Nanjing Medical University, Nanjing, 210029, China
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
167-169
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）