Comparison of Different Universal Primers Used for the Bacterial Community Analyses in Activated Sludge

摘要：

The present study was conducted to compare the specificities of different universal primers used for the analyses of bacterial communities in activated sludge. For the analyses of bacterial communities in activated sludge, PCR-aided methods with universal primers are widely used. Yet, it is anticipated that the specificities of universal primers may be slightly different among the universal primers. Activated sludge taken at Beijing University of Technology were used for the study. DNA was extracted from the samples, and PCR was conducted with thirteen universal primer sets, with either forward or reverse primer labeled with fluorescent dye. The amplicons were digested with rescriction enzymes Hhal, Mspl or Rsal, and the fragments were analyzed. By the comparison of the terminal restriction enzyme fragment length polymorphism(T-RFLP) patterns, it was found that the specificities of the universal primers are similar in general. Yet, minor differences were also found among the universal primer sets.

关键词： PCR T-RFLP universal primers activated sludge bacteria

作者: Aya Tazaki Motoharu Onuki Mamoru Oshiki Hiroyasu Satoh Yong-Zhen Peng Takashi Mino

作者单位: Institute of Environmental Studies, The University of Tokyo Integrated Research System for Sustainability Science, The University of Tokyo College of Environmental and Energy Engineering, Beijing University of Technology

会议类型: 国际会议

会议名称: 第七届中日水环境研讨会暨NSFC-JST重大国际合作项目成果交流会

会议地点: 北京

会议语种:英文

页码: 27-35

在线出版日期: 2007-10-25（万方平台首次上网日期，不代表论文的发表时间）