Substrate Competition between PAOs and GAOs in EBPR A Sludge Processes Analyzed by MAR-FISH
The amounts of acetate uptake by polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) were compared under different pH conditions. These two microorganisms are known to play key roles in the enhanced biological phosphorus removal (EBPR) processes. For the study, microautoradiography (MAR) with acetate as the radiolabeled substrate and fluorescence in situ hybridization (FISH) with PAOmix probe and GB probe were applied. Activated sludge samples taken at a wastewater treatment plant were subjected to anaerobic batch incubation with radiolabeled acetate with different initial pH conditions. The relative amount of carbon uptake by each cell was estimated based on the silver grain area around the cell. The results showed that anaerobic acetate uptake by PAOmix-positive and GB-positive cells were affected by the initial pH. Both PAOs and GAOs were found to have a weak tendency to take up less acetate under higher initial pH conditions.
polyphosphate accumulating organisms glycogen-accumulating organisms enhanced biological phosphorus removal microautoradiography
Kazuya Fujiwara Motoharu Onuki Hiroyasu Satoh Takashi Mino
Institute of Environmental Studies, Univ.of Tokyo Integrated Research System for Sustainability Science, Univ.of Tokyo
国际会议
第七届中日水环境研讨会暨NSFC-JST重大国际合作项目成果交流会
北京
英文
36-43
2007-10-25（万方平台首次上网日期，不代表论文的发表时间）