Comparison of L-ascorbyl-2-monophosphate Na-Ca and L-ascorbyl-2-phosphate-Mg as Vitamin C sources for Yellowtail Seriola quinqueradiata Juveniles
Two feeding experiments were conducted to examine the efficiency of two types of vitamin C(L-ascorbic acid,AsA) derivatives,L-ascorbyl-2-monophosphate Na-Ca(AMP-Na/Ca)and L-ascorbyl-2-phosphate-Mg(AMP-Mg) in terms of growth,AsA concentration in tissues,and hematology in yellowtail Seriola quinqueradiata juvenile(1.10 ± 0.01 g).Stress resistance for the yellowtail fed AMP-Na/Ca was also determined.In experiment 1,six dry pellet diets with different levels of AMP-Na/Ca(0,12,43,88,440,and 881 mg AsA/kg diet) and AMP-Mg(0,16,52,106,595,and 1164 mg AsA/kg diet) were used.Each diet was fed to the yellowtail three times per day for 50 days.In both the vitamin C sources,the survival rates of the fish fed diets without supplemental AsA were lower than 50％ at day 20,and more than 50％ of the fish fed the diet containing 12 or 16 mg AsA/kg died during day 30 to 40,but no significant(P>0.05) differences were detected in survival and growth among the fish groups fed the diets with 43 or 52 mg AsA/kg.Liver and brain AsA concentrations generally increased with increasing dietary AsA level of AMP-Na/Ca or AMP-Mg.In experiment 2,dry pellet diets were formulated to contain 43,88,440,and 881 mg AsA/kg using AMP-Na/Ca and the growth experiment lasted 60 days,and the yellowtails were subjected to low salinity and air dive stress.The fish receiving diets with 440 mg AsA/kg showed significantly(P<0.05) higher tolerance to low salinity stress than those of other groups.The fish receiving diets with 440 mg AsA/kg showed the highest survival rate in air dive stress test among all groups.The present study showed that the juvenile yellowtail could use both AMP-Na/Ca and AMP-Mg as AsA sources.The optimum dietary levels of AMP-Na/Ca and AMP-Mg for the yellowtail juveniles were estimated to be 43-52 mg AsA/kg.Furthermore our results also indicate the necessity of inclusing 440 mg AsA/kg diet for production of yellowtail under stressful conditions.
vitamin C stress tolerance yellowtail Seriola quinqueradiata
Ren Tong-Jun Shunsuke Koshio Shin-ichi Teshima Manabu Ishikawa Arthur Panganiban Jr Md Sham Alam
Science of Marin Resources,The United Graduate School of Agricultural cience,Kagoshima University,1- Laboratory of Aquatic Animal Nutrition,Faculty of Fisheries,Kagoshima University,4-50-20,Shimoarata Science of Marin Resources,The United Graduate School of Agricultural Science,Kagoshima University,1
国际会议
珠海
英文
197-208
2004-09-05（万方平台首次上网日期，不代表论文的发表时间）