会议专题

REMAS: a new regression model to identify alternative splicing events from ezon array data

Background: Alternative splicing (AS) is an important regulatory mechanism for gene expression and protein diversity in eukaryotes. Previous studies have demonstrated that it can be causative for, or specific to splicing-related diseases. Understanding the regulation of AS will be helpful for diagnostic efforts and drug discoveries on those splicing-related diseases. As a novel exon-centric microarray platform, exon array enables a comprehensive analysis of AS by investigating the expression of known and predicted exons. Identifying of AS events from exon array has raised much attention, however, new and powerful algorithms for exon array data analysis are still absent till now.Results: Here, we considered identifying of AS events in the framework of variable selection and developed a regression method for AS detection (REMAS). Firstly, features of alternatively spliced exons were scaled by reasonably defined variables. Secondly, we designed a hierarchical model which can represent gene structure and transcriptional influence to exons,and the lasso type penalties were introduced in calculation because of huge variable size. Thirdly, an iterative two-step algorithm was developed to select alternatively spliced genes and exons. To avoid negative effects introduced by small sample size, we ranked genes as parameters indicating their AS capabilities in an iterative manner. After that, both simulation and real data evaluation showed that REMAS could efficiently identify potential AS events,some of which had been validated by RT-PCR or supported by literature evidence.Conclusions: As a new lasso regression algorithm based on hierarchical model, REMAS has been demonstrated as a reliable and effective method to identify AS events from exon array data.

Hao Zheng Xingyi Hang Ji Zhu Minping Qian Wubin Qu Chenggang Zhang Minghua Deng

LMAM, School of Mathematical Sciences and Center for Theoretical Biology, Peking University, Beijing Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Beijing 100850, China

国际会议

The 7th Asia-Pacific Bioinformatics Conference(第七届亚太生物信息学大会)

北京

英文

185-193

2009-01-01(万方平台首次上网日期,不代表论文的发表时间)