Cleavage in MicroRNA Maturation by Drosha
MicroRNAs are small molecules that are thought to regulate gene expression in many cellular processes such as differentiation,hormone secretion, cancer and infection. They are transcribed from genes that form larger primary (pri-miRNA) transcripts that are then processed by a complex comprised of Drosha and Pasha in the nucleus to generate a smaller fragment (pre-miRNA) which is transported to the cytoplasm. Once in the cytoplasm it is processed by the Dicer protein to generate two smaller complimentary molecules (approx. 21-23 nucleotides in length) of which only one (guide strand) is associated with the RNAinduced silencing complex (RISC). The com plex is then targeted to the complimentary strand within a specific transcript which is then degraded which effectively silences the gene. The structure of the pri-miRNA is important for Drosha cleavage into pre-miRNAs. Recent studies show that the terminal loop structure is required to be at least 10 nucleotides in length for cleavage to occur. The current study aims to determine whether the number and/or size of internal loops or bulges also have a role in Drosha dependant cleavage.
Jiyuan AN Yongjun Fan Anthony Beckhouse Alistair Chalk Christine Wells
The National Centre for Adult Stem Cell Research Griffith University, Australia
国际会议
The 7th Asia-Pacific Bioinformatics Conference(第七届亚太生物信息学大会)
北京
英文
860
2009-01-01（万方平台首次上网日期，不代表论文的发表时间）