Computational evidence of A-to-I RNA editing in the nucleus transcriptome of ArobidopsiS thaliana
RNA editing is a type of post-transcriptional modifications that can increase the complexity of the transcriptome via the insertion, deletion or substitution of single or multiple nucleotides of mRNA sequences. In the last two decades, the A-to-I RNA editing, which is catalyzed by the ADAR family enzymes, was discovered in many metazoans. The C-to-U and U-to-C RNA editing were found in the mitochondria and plastids of plants. However, there is no report of RNA editing in the plant nucleus transeriptome (Table 1). In the current paper, we compared the transcriptome and the genome of Arabidopsis thaliana and use statistical methods to analyze the results. We observed an excessive number of single site A-to-G discrepancies overwhelming any other type of discrepancies between the transcriptome and the genome (Figure I.A). In statistics, this observation can not be explained by either the variation of DNA or the errors of the sequencing experiments. According to the similar reports in human and mouse 1, 2, this observation indicated the existence of A-to-I RNA editing. The tissue dependent distribution of the edited transcripts indicated that the A-to-I RNA editing is related to special physiology process or the special stage of development ofArabidopsis thaliana (Figure 1.B).
Pufeng Du Yanda Li
MOE Key Laboratory of Bioinformatics and Bioinformatics Div.TNLIST / Department of Automation, Tsinghua University, Beijing 100084,China
国际会议
The 7th Asia-Pacific Bioinformatics Conference(第七届亚太生物信息学大会)
北京
英文
863
2009-01-01（万方平台首次上网日期，不代表论文的发表时间）