Order and Content of Thymine in Protein Coding Frames of mRNA Sequences
The order and content of thymine in protein coding frames of mRNAs are playing a major role in translating proteins with adequate hydrophobic residues. Importantly the framel(second nucleotide of codon) prefers to have 27% of thymine, which corresponds to the amount of large hydrophobic residues in the protein. To understand this further at single gene or protein level, this work has been taken up. The gene sequence of tumor suppressor(tp53) protein is chosen for study. The study includes the thymine content in gene and mRNA sequences tp53 of different species.Particularly, the thymine content in all six frames is counted. The amount of thymine in introns and exons are counted separately. The results indicate that the gene contain almost equal amount of thymine and adenine(i.e., thymine in strand2) while it is not so in mRNA. The coding mRNA sequence contains less thymine than adenine. The introns on the other hand contain greater amount of thymine than adenine. The reduction in thymine content in coding frames leads to reduction in number of large hydrophobic residues in the corresponding protein. So the loss in hydrophobicity leads to reduction in activity. Further the carbon distribution analysis was carried out on this protein, which again shows lack of carbon content.
P.Anandagopu E.Rajasekaran
Department of Biotechnology Periyar Maniammai University, Thanjavur -613403,Tamil Nadu, India
国际会议
The 7th Asia-Pacific Bioinformatics Conference(第七届亚太生物信息学大会)
北京
英文
871
2009-01-01（万方平台首次上网日期，不代表论文的发表时间）