Polysilicon Wire Sensor for Biochemical Detection with the Help of Capillary Atomic-Force-Microscopy Tip for Solution Transfer
In this paper,we proposed a polysilicon (poly-Si) wire based biosensor for the detection of glucose and matrix metalloproteinase (MMP) extracted from cancer cells. A focus-ion-beam (FIB) processed capillary atomic-force-microscopy (C-AFM) tip was used to help for transferring trace amount of glucose and MMP solutions onto the exact position of the surface of the poly-Si wire sensor. Glucose solution with different concentrations and MMPs extracted from humans lung adenocarcinoma cells A549 and H1299 were then determined from the current flowing through the poly-Si wire. Due to different amount of hydrogen ions bounded to the poly-Si wire surface when different glucose solutions or MMPs were dropped on the poly-Si wire surface,charge accumulation would occur in the poly-Si wire and causing different current levels been measured. Our experimental data showed that the poly-Si wire sensor is effective and has high sensitivity for glucose and tumor cell MMPs detection when C-AFM tip is used for solution transfer.
You-Lin Wu Po-Yen Hsu Shin-Hsin Hu Wen-Chang Hung Shi-Ting Lin Chung-Ping Hsu A.G.Cullis
Department of Electrical Engineering,National Chi Nan University,Puli,Nantou,Taiwaa Graduate Institute of Biomedicine and Biomedical,National Chi Nan University,Puli,Nantou,Taiwan. Department of Electronic and Electrical Engineering,University of Sheffield,Mappin Street,Sheffield, Division of Chest Surgery,Department of Surgery,Taichung Veterans General Hospital,Taichung,Taiwan
国际会议
9th International Conference on Solid-State and Integrated-Circuit Technology(第9届固态和集成电路国际会议)
北京
英文
2581-2584
2008-10-20（万方平台首次上网日期，不代表论文的发表时间）