Cloning and Characterization of Genes Encoding Glycoside Hydrolase Family 5 Enzymes from the Basidiomycete Phanerochaete chrysosporium

摘要：

Introduction The total genome sequences of the basidiomycete Phanerochaete chrysosporium has been disclosed by the DOE Joint Genome Institute in the United States (http://genom.jgi-psf.org/Phchr1/Phchr1.home.html) and demonstrated the potential as a source to produce various glycoside hydrolases (GHs) acting on lignocellulosic biomass. Most of the enzymes, however, have not been well characterized. Thus, recently, we have decided to clone cDNAs encoding various GHs by using the gene information and to produce the recombinant enzymes as an attempt to improve the enzymatic saccharification process of cellulosic biomass. In this study, we have cloned three cDNAs encoding GH family 5 enzymes from P. chrysosporium and characterized by sequence analysis of them.

作者: Michiko Okamoto Takuya lshida Masao Hiraishi Hitoshi Suzuki Takeshi Tsukada Kiyohiko igarashi Masahiro Samejima

作者单位: Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences,The Universit Department of Biomaterials Sciences, Graduate School of Agricultural and Life Sciences,The Universit

会议类型: 国际会议

会议名称: International Symposium on Wood Science and Technology(IAWPS 2008)(国际木材产品学会联合会2008学术研讨会)

会议地点: 哈尔滨

会议语种:英文

页码: 407

在线出版日期: 2008-09-27（万方平台首次上网日期，不代表论文的发表时间）