Analysis of Factors Affecting the Yield of Enzymatic Synthesis of Ursolic Acid Glycoside Esters by Orthogonal Ezperiment Method
Enzymatic synthesis of ursolic acid glycoside ester (UAGE) from ursolic acid（UA） and glycoside using lipase from Candlda sp was studied. In based on in the orthogonal testing method foundation, the effects of various reaction parameters such as temperature, time, UA concentration, and amount of lipase were studied. The result indicates that the best conditions for UAGE synthesis were as follows: UA concentration 2mol/l, lipase concentration 150g/L, 50℃,.20h. The maximum yield of enzymatic synthesis of UAGE was 32.2%. By variance analysis, temperature and UA concentration have significant influence on yield of UAGE, while time and amount of lipase have not significant influence on yield of UAGE.
Esterification Lipase Ursolic Acid Glucose Orthogonal Testing
ZONG Wei MA Han-jun AN Guang-jie
School of Food and Biological Engineering, Zhengzhou University of Light Industry, P.R.China, 450002 College of Food Engineering, Henan Institute of Science and Technology, P.R.China, 453003
国际会议
2008年国际应用统计学术研讨会(2008 International Institute of Applied Statistics Studies)
烟台
英文
1-3
2008-08-14（万方平台首次上网日期，不代表论文的发表时间）