Induce Apoptosis of Buckwheat Trypsin Inhibitor on Acute Myeloid Leukemia Cells

摘要：

The possible effects of a recombinant buckwheat trypsin inhibitor(rBTI)on the induction of apoptosis of the human HL-60 cell line(acute myeloid leukemia cells)were investigated．Apoptosis of the HL-60 cell line induced by rBTI was identified by MTT(3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide)assays, DNA fragmentation and electrophoresis, flow cytometric analysis and morl)hological observation of nuclei. The results showed that rBTI could specifically inhibit the growth of human acute myeloid leukemia HL-60 cells at a low concentration(1 2．5-100μg/ml)of rBTI in a dose-dependent manner, but there were minimal effects on normal human peripheral blood mononuclear cells(PBMCs). The results indicated that rBTI can induce apoptosis of HL-60 cells and that it might be apotential proteindrug．

关键词： Trypsin inhibitor Common buckwheat HL-60 cells Apoptosis

作者: Zhang Zheng Gao Li Li Yuying Wang Zhuanhua

作者单位: Key Laboratory for Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Key Laboratory for hemical Biology and Molecular Engineering of Ministry of Education, Institute of

会议类型: 国际会议

会议名称: The 10th International Symposium on Buckwheat(第十届国际荞麦会议)

会议地点: 陕西杨凌

会议语种:英文

页码: 196-200

在线出版日期: 2007-08-14（万方平台首次上网日期，不代表论文的发表时间）