Cloning and Sequence Analysis of Gene Fragment of Major Allergenic Protein in Tartary Buckwheat

摘要：

The partial gene of major allergenic protein in tartary buckwheat was amplified from the total RNA of tartary buckwheat using RT-PCR and 5-rapid amplication of eDNA ends(5-RACE)methods. The gene sequence consisted of 1005 nucleotides and a 960 bp open reading frame(ORF)which encoded a protein of 320 amino acids residues．Results ofhomology analysis revealed that the eDNA sequence shared 90%homology with the major allergenic storage protein and legumin-like protein of common buckwheat. The deduced amino acid sequence shared 84%and 76%homology with the legumin-like 13S storage protein and the major allergenic storage protein of common buckwheat respectively. The obtainment of gene fragment of major allergenic protein in tartary buckwheat shouldlay an important foundation for further study of the tartary buckwheat allergen．

关键词： Tartary buckwheat Allergenic protein Clone Sequence analysis

作者: Zhang Xin Zhao Xiaozhen Jing Wei He Dongliang Wang Zhuanhua

作者单位: Key laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China

会议类型: 国际会议

会议名称: The 10th International Symposium on Buckwheat(第十届国际荞麦会议)

会议地点: 陕西杨凌

会议语种:英文

页码: 201-204

在线出版日期: 2007-08-14（万方平台首次上网日期，不代表论文的发表时间）