Proteome Approach as a Tool for the Efficient Separation of Seed Storage Proteins from Buckwheat
The seed storage proteins from the buckwheat were better separated and isolated through the use of two-dimemional electrophoresis(2-DE). The proteins extracted from the whole seed proteins were better separated and observed on the use of lysis buffer．Using this method, the MgMy reproducible isoclcctric focusing(IEF) Can be obtained from polyacrylamide gels, and IEF from the polyaerylamide gel at all the possible pH range(5.0-8.0)was more easily separated than IPG(immobilized pH gradient)gels. The polyacrylamide gels in the first dimension in 2-DE Was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel Was used and the experiment was further investigated under the various conditions．
Seed storage protein Buckwheat Proteom approach Separation
Sun-Hee Woo Hisashi Hiranod Adachi Taiji Keun-Yook Chung Min-Hwa Park Seung-Woo Cho Ki-Na Yun Tae-Seon Kim A-Rum Kim Yong-Gu Cho Beom-Heon Song Chul-Won Lee
Dept.of Crop Science, ChungbukNational University, Cheongju 361-763, KOREA International Graduate School of Arts and Sciences Supramolecular Biology, Yokohama City University, Center for Advanced Science and Innovation, Osaka University, Osaka, 567-0087 JAPAN Dept.of Agricultural Chemistry, Chungbuk National University, Cheongju361.763, KOREA
国际会议
The 10th International Symposium on Buckwheat(第十届国际荞麦会议)
陕西杨凌
英文
205-210
2007-08-14（万方平台首次上网日期，不代表论文的发表时间）