A Primary Ezploration of a Novel Way for Developing Long Repeats SSR Markers of Tartary Buckwheat

摘要：

A genomic library enriched with(gT)n repeats from tartary buckwheat(Fagopyrum tataricum)was constructed using 5-anchored PCR and Dynabeads purifying technology for thedevelopment of long repeats microsattelhte markers．Sequencing analysis of 5 clones from the library showed that they all contained microsatellites in both ends of the insert, and at least one of this two SSR loci have a long repeats in each insert, they could possibly be used for detecting the polymorphism of tartary buckwheat．

关键词： Anchored PCR Dynabeads Tartary buckwheat Microsatellites

作者: Yanqin Li Quanbin Zhang Tonglin Shi Zhongwen Zhang

作者单位: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of IPGRI Office for East Asia, c/o The Chinese Academy of Agricultural Sciences.Beijing, 100081, P.R.Ch

会议类型: 国际会议

会议名称: The 10th International Symposium on Buckwheat(第十届国际荞麦会议)

会议地点: 陕西杨凌

会议语种:英文

页码: 216-219

在线出版日期: 2007-08-14（万方平台首次上网日期，不代表论文的发表时间）