Eztraction, Purification and Characterization of a New Allergenic Protein from Tartary Buckwheat Seeds
An allergenic protein from tartary buckwheat seeds was purified with salting out, anion exchange chromatography and gel filtration. SDS-PAGE analysis and gel filtration showed that the allergenic protein (TBt) was composed of two subunits, we named them TBa and TBb respectively which were linked by disulfide bonds. TBa, TBb and TBt respectively have an estimated molecular weight of 24 kD, 35 kD and 58 kD, and the isoelectric point were about 6.85, 5.85 and 7.00. Immunological character of these proteins were analyzed, and it was found that TBa and TBb had higher IgE binding activity than TBt, so it was believed TBa and TBb both might cause allergy.
Fagopyrum tataricum Eztraction Purification Characterization
Cui Xiaodong Li Fang Li Yuying Wang Zhuanhua Zhang Zheng
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, P.R.China
国际会议
The 10th International Symposium on Buckwheat(第十届国际荞麦会议)
陕西杨凌
英文
492-495
2007-08-14（万方平台首次上网日期，不代表论文的发表时间）