Cloning of cDNA encoding farnesyl diphosphate synthase from the green peach aphid,Myzus persicae
Green peach aphid,Myzus persicae,is a wodd-wide pest infesting different crop plants including cruciferous plants.Most pest aphids use sesquiterpene(E)-beta-farnesene as the major component of the aphid alarm pheromone to signal when a colony is under attack. This compound is produced from famesyl diphosphate(FPP).Many plant genes involved in biosynthesis of FPP have been cloned,but the homologous genes have been rarely cloned from insects.Here two cDNAs(designated as MpFPSh415 and MpFPSb414)encoding famesyl diphosphate synthase(FPS)were cloned from the head and the body of Myzus persicae using Consensus Degenerate Hybrid Oligo Primer(CODEHOP),touchdown polymerase chain reaction (PCR)and nested PCR. They are 415bp and 414bp in length,each with an open reading frame of 123 amino acids.Sequence analyses demonstrated that both of the two deduced peptide sequences contained the conserved domain DDXXD characterized by all FPSs known to date,and they were similar to each other in sequence (Identity=88.62％).Phylogenetic analyses showed that MpFPSh415 and MpFPSlMl4 clustered in the same group as the homologous sequences of other insects(bootstrap score＞70％),but in the different group from that of other taxa.Different FPS cDNAs were cloned from different part of the same insect,indicating that structural variation might have taken place due to functional differentiation. This is the first report on cDNAs coding for insect FPSs to be successfully cloned without previously constructing a cDNA library.
Zheng-Xi Li
Department of Entomology,China Agricultural University,2 Yuanmingyuan West Road,Beijing 100094,China The State Key Laboratory of Integrated Management of Insect and Rodent Pests in Agriculture,Chinese Academy of Sciences,China
国际会议
北京
英文
345-349
2006-10-24（万方平台首次上网日期，不代表论文的发表时间）