Effects of enzyme inhibitors on acetylcholinesterase and detozification enzymes in Propylaea japonica and Lipaphis erysimi
The effects of three enzyme inhibitors on the activities of acetylcholinesterases(AChE),carboxyesterases(CarE)and glutathione-S-transferases(GST),in vivo,were studied in field populations of Propylaea japonica(Thunberg)and Lipaphis erysimi Kahenbaeh collected in Shangjie,Fuzhou,Fujian Province,China.As a specific inhibitor of detoxification enzyme,diethyl maleate(DEM)or triphenyl phosphate(TPP)displayed a strong inhibition on GST or CarE activity in P.japonica and L. erysimi,in vivo. In addition,significant inhibitions on their non-specific targets,i.e.piperonyl butoxide(PB)on GST,CarE or AChE,TPP on GST and AChE,or DEM on CarE and AChE,were also found in this study. In particular,simultaneous multi-attack on the activity of the three enzymes by an enzyme inhibitor WSS found.However,the inhibitions varied depending on the treatment time and dosages of the inhibitors,whenever the inhibitions happened on specific or non-specific targets in the two insect species.From the results obtained in this study,more than one target for PB,TPP or DEM might exist in the two insects,besides the traditional target of the enzyme inhibitors. This could provide a plausible explanation for the improvement in insecticide toxicity by the three enzyme inhibitors in view of the inhibitors multi-attack on the AChE or detoxification enzymes.
Chun Yu Kang Tadashi Miyata Gang Wu
Key Laboratory of Biopesticide and Chemical Biology(Fujian Agriculture and Forestry University),Mini Laboratory of Applied Entomology,Graduate School of Bioagricultural Sciences,Nagoya University,Nagoy
国际会议
北京
英文
350-358
2006-10-24（万方平台首次上网日期，不代表论文的发表时间）