Response surface optimization of medium composition for phenylalanine ammonia lyase production by recombinant E. coli
A culture medium for phenylalanine ammonia lyase (PAL) production in E. coli was developed following preliminary studies by means of response surface methodology. Central composite design (CCD) was utilized to optimize the medium constituents and explain the combined effects of four medium constituents, viz., glucose, yeast extract, (NH4)2HPO4 and MgSO4. The optimum values for the tested varibles were: glucose 28.2 g/l, yeast extract 5.01 g/l, (NH4) 2HPO4 7.02 g/l and MgSO4 1.5 g/l. A second-order model equation was suggested and then validated experimentally. The model adequacy was very satisfactory as the coefficient of determination was 0.94. The maxium PAL production was 65.36 U/g which showed overall 7.9-fold enchancement over the original medium.
Activity Phenylalanine ammonia lyase Recombinant E. coli Response surface method Optimization Culture medium
Jian-dong Cui Yan Li
College of Bioscience and Bioengineering,Hebei University of Science and Technology,186 YuHua East Road,Shijiazhuang 050018,Peoples Republic of China
国际会议
广州
英文
1399-1404
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）