Effects of manual adjusting culture solutions pH of MC1 on lignocellulose decomposing ability of rice straw
In order to enhance the decomposing ability and potentiality of MC1, the culture solution pH was adjusted to 7.0 while it was decreased to 6.5 due to the decomposition of rice straw. The effects on its decomposing capacity and microorganisms were studied through manual adjustment, by measuring the degradation rate of the rice straw, cellulose, hemicelluloses, and the composition and content, microbial composition of each process products. The study indicates that the decomposition rate is up from 33.3％ to 67.5％ after adjusting solution pH with 5％w/v straw treatment. The pH is faster than normal to reach MCls balance point (8.0) after the way of adjusting. For example, the pH of culture solution with 3％w/v straw treatment is already back to 7.0, but the control is 5.6 in normal treatment until the twelfth day. In addition, there is an inhibition effects on the accumulation of water-solubility substances produced during the decomposing process. The content of water-solubility substances with 5％w/v straw control treatment is 12mg/ml, two times higher than that of the modifying treatment. From the DGGE analysis, adjusting pH of the solution enables more microbes of MCl to participate the decomposing of the lignocelluloses, especially the Uncultured Rumen bacterium and Uncultured Clostridiaceae bacterium which are cellulose-decomposing microorganisms. So it is significantly enhanced the decomposing ability and potentiality of MCl after adjusting the pH of the solution.
lignocellulose lignocellulose decomposing composite microbial system MC1, adjustment of pH
Wang Xiaojuan Wang Xiaofen Zhu Wanbin Cheng Xu Guo Peng Cui Zongjun
Center of Biomass Engineering,China Agricultural University/ College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,PR China
国际会议
广州
英文
885-890
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）