Metabolic pathways of hydrogen production in anaerobic activated sludge fermentation process
Bio-hydrogen production from organic wastewater by anaerobic activated sludge fermentation has been investigated widely and it is known that hydrogen could be produced by glucose fermentation through three metabolic pathways, i.e. oxidative decarboxylation of pyruvic acid to acetyl-CoA, oxidation of NADH to NAD+ and acetogenesis by hydrogen-producing acetogens. However, it has not been clear by now which pathway for hydrogen production exactly exist or dominant in anaerobic activated sludge fermentation process. A continuous stirred-tank reactor (CSTR) was introduced and a specifically acclimated fermentative acidogenic microbial microflora obtained under certain operation condition. And then, the hydrogen production activity and the potential hydrogen-producing pathways in the acidogenic microbial microflora were investigated by batch culture in Erlenmeyer flasks with a work volume of 500 mL. With a initial glucose concentration of 10g/L, pH 6.0 and a biomass of 1.01 g/L in terms of mixed liquid volatile suspended solid (MLVSS), about 247.7 mL hydrogen obtained after a 68-hour-cultivation period under temperature 35 ℃. The results of a further test indicated that, among the 247.7 mL hydrogen, about 69％ hydrogen produced from oxidative decarboxylation of pyruvic acid, while 31％ from the oxidation of NADH to NAD+. Hydrogen-producing acetogens did not exist or could not work effectively in the anaerobic activated sludge due to its no contribution to the hydrogen yield.
hydrogen production acidogenic microflora fermentation metabolic pathway NADH/NAD+, acetogenesis
Junguo He Liguo Zhang Jianzheng Li Sheng Chang Zhi Qin
State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 1 School of Chemical and Environmental Engineering,Harbin University of Science and Technology,Harbin,
国际会议
广州
英文
862-868
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）