HYDROLYTIC ACTIVITY OF ALMOND β-GLUCOSIDASE UPON CELLOOLIGOSACCHARIDES
We have made experimental studies into the enzymatic hydrolysis of cellotriose, cellotetraose and cellopentaose at pH 5.0 within the reaction time range of 0 min to 120 min, the temperature range of 30 ℃ to 70 ℃, the β-glucosidase dosage range of 0.0 mg/ml to 0.4 mg/ml, and substrate concentration range 1.0 mg/ml to 3.5 mg/ml. The results indicate that almond β-glucosidase showed higher activity on short cellooligosaccharide than on longer one. The optimal temperature was determined to be 50 ℃. Higher enzyme dosage could improve the hydrolysis of cellotriose and cellotetraose, and inhibit cellopentaose from degrating. It was also found that an increased substrate concentration resulted in decreases in hydrolysis percentage.
Cellooligosaccharides β-glucosidase hydrolysis percentage activity
Hong PENG Lu LIN Yu-huan LIU
State Key Laboratory of Pulp and Papermaking,South China University of Technology,Guangzhou 510640,C State Key Laboratory of Pulp and Papermaking,South China University of Technology,Guangzhou 510640,C State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China
国际会议
广州
英文
835-840
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）