Effects of Microwave/Alkali Pretreatment on Sugarcane Bagasse Enzymatic Hydrolysis and Accessibility
In this study, a novel microwave-assisted alkali pretreatment was used to pretreat sugarcane bagasse (SCB). Compared to traditional alkali pretreatment, microwave-assisted alkali pretreatment had higher rate of weight loss and could increase the cellulose content and decrease the hemicellulose and lignin content. When pretreated at 700 W for 60min, the SCB had the lowest lignin content of 9.69％, hemicellulose of 11.03 ％ and the highest cellulose content of 69.97 ％. The pretreated samples were hydrolyzed by commercial cellulase and the yield of reducing sugar was measured to evaluate the pretreatment effect. When the pretreatment time was 60min, the microwave-assisted pretreated sample at 700 W had the highest reducing sugar recovery rate of 30.8％ after hydrolyzed for 12 h, and the traditional alkali pretreated sample was 23.26 ％, while the untreated samples were only 13.63 ％. Further results suggested that the rate of protein absorption and enzyme absorption increased with the pretreatment time and power, and was higher than the traditional alkali pretreatment. Thus a higher enzyme accessibility could be obtained by this microwave-assisted alkali pretreatment and was consistent with the increase of reducing sugar yield.
Microwave Sugarcane bagasse Hydrolysis Accessibility
Yan-qiu Guo Zhi-wei Lv Jin-shui Yang Hong-li Yuan
College of Biological Science,Key Laboratory of Agro-Microbial Resource and Application,Center of Biomass Engineering,China Agricultural University,Beijing 100193,China
国际会议
广州
英文
822-827
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）