CELLULASE PRODUCTION BY SOLID STATE FERMENTATION USING BAGASSE WITH Penicillium decumbens L-06

摘要：

The cellulase produced by solid state fermentation (SSF) using bagasse with Penicillium decumbens L-06 was investigated in this paper. The optimum conditions for cellulase production by single factor testing were achieved as: the ratio of bagasse to wheat bran 1:1 (W/W), the ratio of water to material 3:1, culture temperature 30℃, initial pH 5.0, ammonium sulfate as nitrogen source with the concentration of 1％, fermentation time 6d. Box-Behnken factorial design (BBD) and response surface methodology (RSM) were further used to optimize conditions for cellulase production. The maximal cellulase (FPA activity) production (3.89 FPU g-1) was obtained under the optimized conditions (ratio of water to material 2.38:1, initial pH 5.28, cultivation time 150.5 h). Its well corresponded to the calculated results (3.97 FPU g-1 ) by model prediction.

关键词： Penicillium decumbens Solid-state fermentation Cellulase Bagasse Box-Behnken factorial design Response surface methodology

作者: Chuan-nan Long Yue-qin Ou Ping Guo Yun-tao Liu Qiu-rong Huang Jing-jing Cui Min-nan Long Zhong Hu

作者单位: Department of Biology,Shantou University,Shantou 515063,China The School of Energy Research,Xiamen University,Xiamen 361005,China

会议类型: 国际会议

会议名称: 2008年生物质能源技术国际会议

会议地点: 广州

会议语种:英文

页码: 751-760

在线出版日期: 2008-12-03（万方平台首次上网日期，不代表论文的发表时间）