Effect of pH on biohydrogen production by anaerobic mixed microflora
Experiments were conducted to investigate H2 production from glucose by natural anaerobic sludge at various pH. H2 production clearly increased with increasing pH from 4.0 to 6.0, and then decreased when pH increased from 6.0 to 8.0. The maximum H2 yield was obtained at pH 6.0. H2 production, specific H2 production rate, and the ratio of butyrate/acetate (HBu/HAc) followed a similar trend, suggesting that the formation of butyrate may favor H2 production. A modified Gompertz model adequately described H2 production and bacterial growth. Energy analysis revealed that the percentage of gaseous energy recovery obtained using mixed microflora and considering glucose as a substrate was 26.43％. Using 16S rDNA genes separated by denaturing gradient gel electrophoresis, the species composition of the mixed microflora was accessed, and most species were found to be affiliated with the genera Clostridium and Bacillus. However, the microbial species varied with pH.
Sludge Hydrogen production pH DGGE Microbial community
Gui-Lan Tang Zhen-Jun Sun Jian Huang Qin-qing Tang
College of Resources and Environmental Sciences,China Agricultural University,Beijing 100094,China Medical School,Nanjing University,Nanjing 210093,China
国际会议
广州
英文
725-734
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）