PREPARATION AND PROPERTIES OF REVERSIBLY SOLUBLE-INSOLUBLE IMMOBILIZED CELLULASE ON EUDRAGIT L-100
pH-responsive immobilized cellulase was prepared by noncovalent and covalent methods. Covalently immobilized celluase with carbodiimide showed more excellence on both immobilization efficiency and reusability than noncovalently immobilized cellulase by adsorbility. NHS (N-hydroxysuccinimide could be used to improve efficiency of covalent coupling that the retained activity of the immobilized cellulase could improve 13.76％ (with and without of the enhancer was 84.21％ and 70.45％, respectively.).The immobilized cellulase had broader optimum temperature between 55 and 60℃ than free cellulase (the optimum temperature was 55℃). The optimum pH values of free and immobilized ceilulase were 4.75, 5.25, respectively. Immobilized cellulase could retain higher activity than free cellulase above pH 5.0. The Km value of the immobilized cellulase and free celluase was 85.55 and 73.84 g·L-1 respectively. The Vmax of immobilized cellulase showed a slight decrease from 2.49 (for free cellulase) to 2.34 μmolml-1·min-1. There was about 50％ of remained productivity after 5 cycles for hydrolysis of both microcrystal cellulose and alkali-pretreated straw.
Immobilized Cellulase Eudragit L-100 NHS
Yu ZHANG Jing-Liang XU Zhen-Hong YUAN Xin-Shu ZHUANG Dong LI
Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences Key Laboratory of Renewable Ene Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences Key Laboratory of Renewable Ene
国际会议
广州
英文
619-625
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）