STUDY ON SYNTHESIS OF FATTY ACID ETHYL ESTERS FROM SOYBEAN OIL BY IMMOBILIZED LIPASE Candida sp. 99-125
In this study, fatty acid ethyl esters (FAEEs) were synthesized from soybean oil by the immobilized lipase Candida sp.99-125. Different conditions such as water content, solvent content, enzyme amount and temperature on the esterification reaction were studied during the optimizing process. The maximum yield was obtained under following conditions: 250μl water content (12.5w.t％ based on the oil weight), 6ml solvent, 0.4g lipase and temperature 40℃. With three-step ethanol added into the reaction system, the best yield of 96.8％ was achieved after totally 12 hours transesterification, i.e. almost all the soybean oil was converted into FAEEs catalyzed by the lipase. Moreover, the lipase can be used for 168 hours continuously with high conversion (around 80 ％ or even higher). The conclusion illustrated that the immobilized lipase from Candida sp.99-125 can efficiently catalyze alcoholysis of soybean oil into FAEEs under appropriate conditions, and it showed great potential for the synthesis of biodiesel in industrial production.
biodiesel ethanol FAEEs lipase optimization
Ji-ke LU Ji-xian HOU Kai-li NIE Fang WANG Tian-wei TAN
Beijing Key Laboratory of Bioprocess,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing,100029,PR China
国际会议
广州
英文
499-504
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）