PSEUDOMONAS FLUORESCEN LIPASE IMMOBILIZED ON LUFFA SPONGE AND ITS APPLICATION IN PREPARING BIODIESEL
Pseudomonas fluorescen lipase was immobilized onto luffa sponge in the medium of N-hexane. The immobilization yield was more than 95.5％ during 30min and corresponds to the loading of 500U/g support. Immobilized pseudomonas fluorescen lipases were used to prepare biodiesel from waste edible oil in solvent-free system, and the methyl ester yield was more than 88.7％ at the temperature of 40℃ with the adding ratio of methyl/oil 3:1. It was found that the catalyzed activity of immobilized pseudomonas fluorescen lipase was similar to the Novozym435 lipase. The residual activity of immobilized pseudomonas fluorescen lipase was more than 80％ after reusing 10 times.
Immobilized lipase Luffa sponge Biodiesel
Fei QIAN Bao-hua ZHANG Jun-dan YE Bin CHEN Xian ZHU Xiao-ya GUO
College of Environmental and Chemical Engineering,Shanghai University,Shanghai 201800,China College of Environmental and Chemical Engineering,Shanghai University,Shanghai 201800,China;Key labo
国际会议
广州
英文
487-491
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）