THE NEGATIVE EXPRESSION OF PEPC GENE IN CYANOBACTERIA TO IMPROVE THE PRODUCTIVITY OF BIODIESEL MATERIAL
The contents of lipids based on biomass are higher than in higher plants, and the accumulation of biomass is much more rapid than in higher plants as well. For improving the productivity of biodiesel material this work tried to increase the content of lipids in cyanobacteria by negative expression of PEPC with antisense technique. The conservative sequence of PEPC from Anabaena (Nostoc) sp PCC 7120 was selected as the target gene fragment (pepcA), which was inserted into the shuttle expression vector pRL-489 in forward or reversely orientation to form pRL-Sen pepcA and pRL-Anti pepcA. The recombinant expression vectors were transferred to Anabaena sp. PCC 7120. Comparing with wild type cells the PEPC enzyme activity of transgenic Anabaena with pRL-Anti pepcA decreased 87.1％, the synthesis of lipids increased 54.7％; While the PEPC enzyme activity of transgenic Anabaena with pRL-Sen pepcA increased 81.1％, and the lipids content decreased 58.4％; These data has not been seen before and may open a new approach to make biodiesel from microaigae.
Phosphoenolpyruvate Carboxylase (PEPC) Negative expression Biodiesel Antisense RNA
Li-Jun HOU Ding-Ji SHI Ze-Fu CAI Xiao-Hui JIA Xue-kui WANG
School of Marine Sciences & Engineering,Tianjin University of Sciences & Technology,Tianjin 300457,C School of Marine Sciences & Engineering,Tianjin University of Sciences & Technology,Tianjin 300457,C School of Marine Sciences & Engineering,Tianjin University of Sciences & Technology,Tianjin 300457,C
国际会议
广州
英文
448-451
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）