Effects of EMP on Bonding Ability of Insulin and its Receptor
AIM To discuss the effects of EMP on bonding ability of insulin and its receptor.METHODS HL-7702L-02GNHu6 cells (5×55-1) were divided into 2 groups: anti-insulin-fluorescent labeling group (including cell-irrad + insulin-irrad group,cell-irrad + normal insulin group,normal cell + insulin-irrad group,normal cell + normal insulin group,total 4 sub-groups) and anti-insulin receptor-fluorescent labeling group (including cell-irrad group,normal cell group,total 2 sub-groups).Some 0.5mg·mL-1 insulin solution and the groups which need to be irradiated were irradiated by 400kV·m-1 EMP.After the irradiation,the indirect immuno fluorescent experiment was done,immediately.And then,all the samples were detected by FCM.RESULTS After EMPs irradiation,the percentage of the L-02 cells which were fluorescent-labelled by FITC on cell membranes insulin receptor increased. After EMPs irradiation,the percentage of the L-02 cells which were fluorescent-labelled by FITC on cell membranes insulin increased than un-irradiated,no matter whether the insulin which was put into the L-02 cells was irradiated or not. Compared with the un-irradiated insulin,no matter the irradiated insulin was put into un-irradiated or irradiated L-02 cells,the percentage of the L-02 cells which were fluorescent-labelled by FITC on cell membranes insulin decreased.CONCLUSION 400kV·m-1 EMP irradiation may probably change insulins spatial structure,decrease the bonding ability between insulin and its receptor,and show some excitatory effect on L-02 cells.
CHEN Yong-Bin LI Jing GUO Guo-Zhen MIAO Xia ZENG Li-Hua Liu Xiao-Wei WANG Xiao-Wu WANG Jin LI Kang-Chu XIE Xue-Jun REN Dong-Qing
Department of Radiation Medicine,Faculty of Preventive Medicine,Fourth Military Medical University,X Department of Nephrology,Xijing Hospital,Fourth Military Medical University,Xian 710033 China
国际会议
昆明
英文
2008-11-01（万方平台首次上网日期，不代表论文的发表时间）