Coproduction of Microbiodiesel and Arachidonic Acid from Microbial Oil
In this work, the microbial oil extracted from Mortierella alpina I502-8 was prepared as the feedstock of microbiodiesel. The microbial oil is rich in polyunsaturated fatty acids (PUFAs), especially Arachidonic acid (ARA). The yield of the biodiesel produced under optimal condition was up to 95％ through alkaline-catalyzed transesterification of the microbial oil. The optimal reaction variables were methanol/oil molar ratio (6:1), KOH concentration (1.0％), temperature (55℃ ), rate of stirring (500rpm), and reaction time (50min). The ARA methyl esters were separated by multi-step dry fractionation in order to reduce the cost of microbiodiesel production. About 86.82％ ARA methyl esters of transesterification product (FAMEs) was separated into L4 (liquid fraction at 4℃ ) after multi-step dry fractionation. S4 (solid fraction at 4℃ ) was used as the microbiodiesei, taking up 7.64％ in total FAMEs and exhibiting the main fuel features defined by Chinese Standards for biodiesel 100 established according to ASTM D6751-2003.
microbial microbiodiesel transesterification Arachidonic acid Multi-Step Dry Fractionation coproduction
La-mei ZHANG La-mei ZHANG Cheng-ling YUAN Juan LI Zhi-ming ZHENG
Key Laboratory of Ion Beam Bioengineering,Chinese Academy of Sciences,Hefei,230031,Peoples Republic of China
国际会议
广州
英文
265-272
2008-12-03（万方平台首次上网日期，不代表论文的发表时间）