A Novel Spectrophotometric Assay for Superoxide Radical Anion Quantification

摘要：

Nitro Blue Tetrazolium(NBT)is a widely used chemical for the determination of superoxide anion radicals(O2-)and superoxide dismutase activity in many fundamental processes.Its reduction reaction with superoxide was explored in this paper as well as the spectrometric characteristics of its reduction products such as monoformazan and diformazan.The molar extinction coefficients of these products in DMSO have been calculated as 120,000 and 235,000 M-1 cm-1,respectively.We propose that this method can be used to determine superoxide anion radical concentration or as a general screening protocol for the efficacy of an antioxidant.

关键词： Nitrotetrazolium Blue Chloride Monoformazan Diformazan Superoxide Anion Radical Absorbance Spectroscopy

作者: LIU RUI-HENG FU SHI-YU ZHAN HUAI-YU

作者单位: State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou,Guangdong Province,510640,China

会议类型: 国际会议

会议名称: 第二届国际造纸与环境学术会议(The Second International Papermaking & Environment Conference)

会议地点: 天津

会议语种:英文

页码: 190-193

在线出版日期: 2008-05-14（万方平台首次上网日期，不代表论文的发表时间）