Proteomic Analysis of Pyrene Stress-Responsive within Plant Roots

摘要：

Phytoremediation is a new method for removing polycyclic aromatic hydrocarbons from the environment.A study was conducted to investigate accumulation of pyrene and protein changes in maize of Zea mays L responding to the stress of 1-week exposure to pyrene in the growth media.A group of three 5-week maize plants was allowed to grow in a nutrient solution of different pyrene concentration(0,0.1,1,and 10mg/L)for 1 week,the roots were then taken for proteomic analysis.Total proteins in the roots were extracted and separated by two-dimensional gel electrophoresis;28 protein spots were difierent from those of the control sample.Further comparisons of isoelectric point,molecular weight and abundance showed 14 up-regulated protein spots,four newly induced protein spots,6 down-regulated protein spots,and lost four proteins.The ten proteins of different molecular weight were subjected to trypsin digestion and then peptide separation by MALDI-TOF mass spectrometry;only six of them matchedthe database at molecular weights of 12.69,21.89,22.30,43.14, 57.22 and 102.61 kDa.The six matched proteins were centromere protein-like protein,putative heat shock protein,putative GTP-binding protein,6-phosphofructo-2-kinase,cytochrome P450,and fructose-2,6-bisphosphatase.The study results have demonstrated the benefits of the proteomic approach in phytoremediation of PAHs and also provided new insights into pyrene degradation related proteins in maize roots.

关键词： proteomic maize pyrene accumulation stress response

作者: XU Shengyou CHEN Yingxu RUAN Aidong ZHANG Wei LI Feng CHEN Xincai

作者单位: Resources and Environmental Engineering Institute,East China University of Science and Technology,Sh Department of Environmental Engineering,Zhejiang University,Hangzhou 310029,China State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University,Nanjing Resources and Environmental Engineering Institute,East China University of Science and Technology,Sh

会议类型: 国际会议

会议名称: The 2nd International Conference on Asian-European Environmental Technology and Knowledge Transfer(第二届亚欧环境技术及知识转化国际研讨会)

会议地点: 合肥

会议语种:英文

页码: 450-456

在线出版日期: 2008-06-05（万方平台首次上网日期，不代表论文的发表时间）