Isolation and Identification of the Strain KW2 Producing Alkaline Cellulase

摘要：

A bacterial strain(designated KW2).which has high alkaline cellulase activity,was isolated from compost samples.Cells were Gram-positive,rod-shaped and spore forming.Strain KW2 was identified as a strain of Bacillus subtilis based on phenotypic,physiological and biochemical characteristics,as well as 16S rDNA phylogenetic analysis.The cellulase activity(CMCase)of shaking flask culture has reached 236 U/m1.The characteristics of the enzyme showed that the optimum pH value of the strain was 8 and the optimum temperature was 55℃.1t had both,good alkaline endurance and stable enzymatic activity at pH value 5.0-9.0 and had remarkable heat stability under 65℃.The cellulase produced by KW2 may be candidates for use in detergents,treating textiles.

关键词： alkaline cellulase isolation identification Bacillus subtilis 16S rDNA

作者: KAN Jinsong LUO Jianping WU Ke YU Zhimin JIN Jie

作者单位: School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China;Depar School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China Department of Biology and Environmental Engineering,Hefei University,Hefei 230022,China

会议类型: 国际会议

会议名称: The 2nd International Conference on Asian-European Environmental Technology and Knowledge Transfer(第二届亚欧环境技术及知识转化国际研讨会)

会议地点: 合肥

会议语种:英文

页码: 94-96

在线出版日期: 2008-06-05（万方平台首次上网日期，不代表论文的发表时间）