Enhanced Production of Laccase from Flammulina velutipes and Its Application in Biobleaching of Kraft Bamboo Pulp
Laccase has been produced from Flammulina velutipes in liquid substrate fermentation using potato juice and glucose as the prime substrates.The nutrient conditions on the production of laccase,such as nitrogen sources and its amount,trace elements of Cu2+ and Mn2+,were optimized in this study.The maximum laccase yield is over 26 IU/mL.Combined with HBT,the laccase was applied to Totally Chlorine Free(TCF)and Elemental Chlorine Free(ECF)bleaching of Kraft bamboo pulps.The results indicated that Kraft bamboo pulps can be bleached to a brightness of 83.1％ ISO using TCF bleaching and 85.8％ ISO using ECF bleaching sequence in combination of LMS treatment.Also the physical properties of paper were improved after LMS bleaching.The ECF bleaching pulps had slightly lower burst index and tensile index,but higher brightness than those of the TCF bleached pulps.Also the enzyme-assisted bleaching before QP stage is proved to be more efficient than its use as a pre-bleaching agent.
nutrient conditions laccase/mediator system(LMS) kraft bamboo pulp totally chlorine free (TCF) elemental chlorine free (ECF)
MENGRU LIU HUAIYU ZHAN SHIYU FU HAO LIU HAILONG LI
State Key Laboratory of Pulp and Paper Engineering,South China University of Technology,Guangzhou,510640,China
国际会议
第二届国际造纸与环境学术会议(The Second International Papermaking & Environment Conference)
天津
英文
419-423
2008-05-14（万方平台首次上网日期，不代表论文的发表时间）