Studies on SNPs of BRCA1 and P53 genes related to breast carcinoma
To investigate the possible implication of the substitution at SNPs sites in BRCA 1 and P53genes in the carcinogenesis of breast cancer,the SNPs in BRCA1 and P53 gene regions from 30 breast cancer patients were analyzed by polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and subsequent DNA sequencing.The result shows that the total ly 6 nucleotide substittution sites at the regions of intron 1 and intron 7 of P53 detected were found in 30 breat cancer patients,which involve 3 published SNPs sites and 3 uncharacterized sites.Among these substitutions,a G→A mutation which occurred at the intron 7 is coupled with the cystidine nucleotide at a SNPs site 20 nucleotides upstream of it which occupies 20％ of the breast cancer patients,implicaing the significance of the coupling of these two sites in the carcinogenesis of the breast cancer.However,the substitution rate at BRCA1 10th exon region is only 2/30 in the patients which is not significantly different from the normal population,suggesting the irrelative of this site to the cancer.The DNA substitution analysis at the SNPs sites of P53 may serve as a helpful tool for the early diagnosis of breast cancer.
Breast cancer BRCA1 gene P53 gene SNPs PCR-SSCP
Ping Wang Shan Wang Yu Li Yubao Zhang
Affiliated Tumor Hospital of Harbin Medical University,Harbin,China 150086 Department of Life Science and Engineering,Harbin Institute of Technology,Harbin,China 150086
国际会议
哈尔滨
英文
330-333
2008-01-13（万方平台首次上网日期，不代表论文的发表时间）