Developmental Activity Changes in P19EC-derived Neurons Cultured on Microelectrode Array

摘要：

One of the major problems in regenerative medicine is its difficulty in evaluation of differentiation process of undifferentiated cells.In this study,P19 embryonal carcinoma (EC) cells were cultured on 64-embedded microelectrode arrays (MEA) and were differentiated into neuronal tissues by application of retinoic acid.The major advantage of MEA system is its noninvasiveness which allow long-term recording of excitable cells.Developmental changes in electrical properties and intracellular Ca2+ oscillations in Pl9EC-derived neuronal tissues during differentiation processes were obtained and analyzed.

关键词： neuronal differentiation P 19 embryonal carcinoma cells microelectrode array

作者: Yuzo Takayama Atushi Saito Hiroyuki Moriguchi Yasuhiko Jimbo

作者单位: University of Tokyo,5-1-5 Kashiwanoha,Kashiwa-shi,Chiba,277-8563,Japan

会议类型: 国际会议

会议名称: 2008国际生物生理工程研讨会(The International Symposium on Biological and Physiological Engineering/The 22nd SICE Symposium on Biological and Physiological Engineering)

会议地点: 哈尔滨

会议语种:英文

页码: 163-164

在线出版日期: 2008-01-13（万方平台首次上网日期，不代表论文的发表时间）