Application of Surface Plasmon Resonance Biosensor in Detecting 3-Nitro-tyrosine

摘要：

This paper describes the sensing characteristics of a surface plasmon resonance(SPR)immunosensor based on self-assembled monolayer(SAM)for detection of 3-nitro-L-tyrosine(3-NT).The inhibition of SPR response increased with the concentration of 3-NT in sample solution.The immunosensor chip showed a low detection limit of 2 ng/ml under 20-min sampling of Ab(3-NT).Working concentration range of the sensor system covers a wide concentration region from 2 to 1000 ng/ml.Ab(3-NT)bound to the sensor chip during an immunoassay could be detached by three regeneration solutions.0.1 M NaOH solution with 0.05 ％ Triton X-1O0 showed the best regeneration performance.

作者: Xiaoyu Ding Jing Jin Yi Tao Huihua Deng Zuhong Lu Hua Lv

作者单位: State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeas State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeas Key Laboratory of Child Development and Learning Science(Southeast University),Ministry of Education

会议类型: 国际会议

会议名称: 2008亚太心智、脑和教育学术会议(Asia-Pacific Conference on Mind Brain and Education 2008)

会议地点: 南京

会议语种:英文

页码: 362-364

在线出版日期: 2008-10-25（万方平台首次上网日期，不代表论文的发表时间）