Determination of 3-Nitro-L-Tyrosine by SPR Immunosensor Based On Self Assembled Monolayer

摘要：

This paper describes the fabrication and sensing characteristics of a surface plasmon resonance(SPR)immunosensor based on self-assembled monolayer (SAM)for detection of 3-Nitro-L-tyrasine(3-NT).The functional sensing surface was fabricated by theimmobilization of a 3-NT-bovine serum albumin(3-NT-BSA)on Au-thiolated SAMs. The fabricated immunosensor demonstrates a detection limit of 2 ngmL-1.Antibody bound to the sensor chip during an immunoassay could be detached by a brief exposure to Triton X-100(volume fraction 0.05％ in 0.1 M NaOH solution.The same sensor chip was used for more than 20 cycles with this surface regeneration regent.

作者: Jing Jin Hua Lv Yi Tao Xiaoyu Ding Dacan Yang Huihua Deng Zuhong Lu

作者单位: Key Laboratory of Child Development and Learning Science(Southeast University),Ministry of Education Chien-Shiung Wu Laboratory,School of Biological Science and Medical Engineering,Southeast University Key Laboratory of Child Development and Learning Science(Southeast University),Ministry of Education

会议类型: 国际会议

会议名称: 2008亚太心智、脑和教育学术会议(Asia-Pacific Conference on Mind Brain and Education 2008)

会议地点: 南京

会议语种:英文

页码: 283-287

在线出版日期: 2008-10-25（万方平台首次上网日期，不代表论文的发表时间）