Study on S180 Tumor Mice Erythrocyte Membrance Function of Sargassum Fusiform Polysaccharides
Objective To study the effect of Sargassum fusiforme polysaccharides (SFPS) on biochemistry function of erythrocyte membrane of S180 mice.Methods A tumor mouse model was developed,and the mice were treated with high,medium,and low doses of SFPS through abdominal injection for 7 d.Erythrocytes were collected and erythrocyte suspensions were prepared.Changes in Ca2+ in the erythrocytes in the tumor-bearing mice were measured using laser confocal scanning; changes in the content of sialic acid and the acitivities of Na+,K+-ATPase and Ca2+,Mg2+-ATPase were meausred using relevant test kits; changes in the level of erythrocyte membrane potential were analyzed using flow cytometry; and changes in the complex mobility of erythrocytes were measured using high performance capillary electrophoresis (HPCE).Results SFPS lowered the intracelluar Ca2+ of erythrocytes in tumor-bearing mice,increased the content of sialic acid on surface of erythrocyte membrane,increased the acitivities of Na+,K+-ATPase and Ca2+,Mg2+-ATPase,and raised the level of erythrocyte membrane potential and the complex mobility of erythrocytes in elelctrophoresis.Conclusions Sargassum fusiforme polysaccharides (SFPS) can adjust or restore some biochemistry functions of erythrocyte membrane of S180 mice.
Sargassum fusiforme polysaccharides (SFPS) erythrocyte membrane biochemistry
Yubin Ji Chenfeng Ji Chong Wang
Postdoctoral Programme,Institute of Meteria Medica,Center of Research and Development on Life Sciences and EnvironmentSciences of Harbin University of Commerce,Harbin 150076,China
国际会议
7th Asian-Pacific Conference on Medical and Biological Engineering(第七届亚太地区生物工程学术会议)
北京
英文
2008-04-22（万方平台首次上网日期，不代表论文的发表时间）