Study of Solanine on Mitochondrion in HepG2 Cell
Objective:To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and Ca2+i in the cells,and to uncover the mechanism by which solanine induces apoptosis.Methods:HepG2 cells are double stained with AO/EB,and morphological changes of the cells are observed using laser confocal scanning microscopy (LCSM).HepG2 cells are stained with TMRE,and change in the membrane potential of mitochondria in the cells are observed using LCSM.HepG2 cells are double stained with Fluo-3/AM,and change of Ca2+i in the cells are observed using LCSM.HepG2 cells are double stained with TMRE and Fluo-3/AM,and both the change in membrane potential of mitochondria and that of Ca2+i in the cells are observed using LCSM.Results:Cells in treated groups show typical signs of apoptosis.The results of staining with TMRE show that solanine can lower membrane potential; those of staining with Fluo-3/AM show that solanine can increase the concentration of Ca2+ in tumor cells; and those of double staining with TMRE and Fluo-3/AM show that solanine can lower membrane potential; those of staining with TMRE show that solanine can increase the concentration of Ca2+ in the cells at the same time as it lowers the membrane potential of mitochondria.Conclusions:Solanine opens up the PT channels in the membrane by lowering the membrane potential,leading to Ca2+ being transported down its concentration gradient,which in turn leads to the rise of the concentration of Ca2+ in the cell,turning on the mechanism for apoptosis.
Solanine Hepatocarcinomatic cell Ca2+ in the cell Membrane potential Laser confocal scanning microscopy (LCSM)
Shiyong Gao Yubin Ji Chenfeng Ji Xiang Zou
Postdoctoral Research Station,The Institute of Materia Medica,Harbin University of Commerce,Harbin 150076,Heilongjiang Province,P.R.of China
国际会议
7th Asian-Pacific Conference on Medical and Biological Engineering(第七届亚太地区生物工程学术会议)
北京
英文
2008-04-22（万方平台首次上网日期，不代表论文的发表时间）