Synthesis and Gene Transfection Efficiency of PEG-chitosan-PEI copolymers

摘要：

PEG-chitosan-PEI copolymer was synthesized by ring-opening reaction of ethylenimine monomers with PEGchitosan.PEG-chitosan was prepared before by an imine reaction between chitosan and mPEG-CHO.With the aim to investigate the gene transfection properties of PEG-chitosan-PEI,we investigated its cytotoxicity by MTT assay,DNA compaction ability by agarose gel retardation,and transfection efficiency of pEGFP in vitro.The results showed higher transfection efficiency and lower cytotoxicity of PEG-chitosan-PEI than PEI(25k) which is a popular gene vector.

关键词： Chitosan PEI PEG Gene vector Gene delivery

作者: Wei Zhang Shi-rong Pan Yu-ting Wen Xin Luo

作者单位: First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,China School of Pharmaceutical Science,Sun Yat-Sen University,Guangzhou,China

会议类型: 国际会议

会议名称: 7th Asian-Pacific Conference on Medical and Biological Engineering(第七届亚太地区生物工程学术会议)

会议地点: 北京

会议语种:英文

在线出版日期: 2008-04-22（万方平台首次上网日期，不代表论文的发表时间）