The vitro and vivo study of Poly (3-hydroxybutyrate) microspheres
Purpose:A controlled-release drug delivery of bovine serum albumin(BSA) has been developed by successful encapsulation of BSA in Poly (3-hydroxybutyrate) (PHB) microspheres.The vitro and vivo characteristics of the PHB microspheres have been investigated.Materials and Methods:BSA-loaded PHB microspheres with a mean size of 6.9-20.3μm were prepared by using the water/oil/water doubleemulsion solvent evaporation method.Results :The diameter of the microspheres ranged from 6.9 to 20.3μm depending on the different preparation parameters.The maximum and minimum BSA encapsulation efficiency within the polymeric microspheres were 69.8 and 7.5％,respectively,varying with preparation conditions.The controlled release characteristics of the microspheres for BSA were investigated in pH 7.4 media.The initial BSA burst release from 8.9 to 63.1％ followed by constant slow release for 28 days was observed for BSA from BSA-loaded microspheres and followed the Higuchi matrix model.The cytotoxicity degree was 1 score when the leaching liquor concentrarions were 100％ and 75％,and the cytotoxicity degree was 0 score when the leaching liquor concentrarions were 50％ and 25％.The inflammatory reaction was heavy on week 1 subcutaneous postimplatation,thereafter,the inflammatory reaction gradually reduced with time.CONCLUSION:The BSA controlled release from microsphere for 1 month could be obtained,demonstrating that the microsphere might be used as the carrier of drug controlled release.Both cellular compatibilty and histological compatibility were considered to be excellent,implying that the microsphere was biocompatible with the body.Significance:The vitro and vivo characteristics of PHB microspheres showed the feasibility of BSA-loaded microspheres as controlled release devices.
vitro cytotoxicity histological examination controlled release Poly (3-hydroxybutyrate) microspheres BSA
Feng Tian Yong-Liang Zhao Chang-Jun Liu Fan Li Nan Xing
Institute of Medical Equipment,Academy of Military Medical Science,Tianjin 300161,China
国际会议
7th Asian-Pacific Conference on Medical and Biological Engineering(第七届亚太地区生物工程学术会议)
北京
英文
2008-04-22（万方平台首次上网日期，不代表论文的发表时间）