Differentiation of the Human Marrow Mesenchymal Stem Cells into Vascular Endothelium-like Cells in vitro
Aim:The two main obstacles of successfully grafting tissue engineered skin are the shortage of the resource of the seed cell and the long process of vascularization of grafted skin.This article investigates the feasibility and condition of differentiation of the human marrow mesenchymal stem cells (MSCs) into the vascular endothelial-like cells in vitro.Methods:The MSCs were harvested ,isolated and purified by combining density gradient centrifugation with adhering method.The cultured cells in induce group were treated with 10μg/L vascular endothelial growth factor(VEGF) and 5μg/L basic fibroblast growth factor(bFGF).No VEGF and bFGF were added in control group.The morphological changes of the cells were observed with inverted phase contrast microscope.The expression of CD34,Ⅷ factor were determined by the immunocytochemistry methods.The transmission electron microscope was used to further explore the ultrastructure characteristics of the cultered cells.Results:The cells induced by VEGF and bFGF showed the morphological changes from long fusiform-shaped to short fusiform or flat shape,which was consistent with the significant characteristic of endothelium cells.The expression of CD34,Ⅷ factor were positive in the induced cells,which were negative in the control group.The Weibel-Palade body could be found in the cytoplasm of some induced cells.Conclusion:Combined VEGF with bFGF can induce MSCs differentiate into vascular endothelial-like cells in vitro,which may be unfold the potential applied prospects for vascularization and application of tissue engineered skin.
mesenchymal stem cells vascular endotheliallike cells vascular endothelial growth factor basic fibroblast growth factor
Dewu Liu Xiangrong Zhang Xiaoliang Li Zhian Zhang Guanghua Guo
Burn center,The first affiliated hospital of Nanchang University.Nanchang 330006,Jiangxi Province,P. Burn center,The first affiliated hospital of Nanchang University.Nanchang 330006,Jiangxi Province,P.
国际会议
7th Asian-Pacific Conference on Medical and Biological Engineering(第七届亚太地区生物工程学术会议)
北京
英文
2008-04-22（万方平台首次上网日期，不代表论文的发表时间）