Effects of Residual Moisture Content on Lyophilized Red Blood Cells
Freeze-drying is one potentially ideal approach for long-term preservation of Red Blood cells (RBCs). Residual moisture content is a crucial factor that influences the stability of freeze-dried RBCs. Effects of residual water moisture on numerical recovery, hemoglobin recovery, SOD and ATP enzyme activities of lyophilized RBCs were studied in this study. The results showed that the numerical and hemoglobin recovery of freeze-dried RBCs remained higher than 60％ when residual moisture content is above 5％, while decreased to 39.4％ and 35.2％ when residual moisture content decreased to 1.5％. Activities of superoxide dismutase (SOD) and adenosine triphosphate (ATP) in freeze-dried RBCs maintained close to those of fresh RBCs when residual moisture content varied from 3.5％ to 7.5％, while decreased sharply to 55.3％ and 46.2％ when residual moisture content declined to 1.5％. The results demonstrated lyophilized RBCs have a minimum moisture content necessary for preventing injury. Overdrying to less than 5％ water content would result in more injury in RBCs membrane and endogenous proteins.
Zhou X L He H Liu B L Hua T C Chen Y
Institute of Biothermal Sciences,University of Shanghai for Science and Technology,Shanghai 200093,P Institute of Blood test,Shanghai Antu Hospital,Shanghai 200093,P.R.China
国际会议
上海
英文
936-939
2008-04-05（万方平台首次上网日期，不代表论文的发表时间）