Dynamic Study of the Effect of Electroacupuncture on Dopamine in the Striatum for Rats with Cerebral Ischemia and Reperfusion
Object:To observe the dynamic changes of dopamine(DA)in the striatum with the process of duplicating cerebral ischemia and reperfusion(CIR)model on rats.And observe the effect of electroacupuncture(EA)on such changes.Methods: Using microdialysis sampling,high performance liquid chromatography(HPLC) detecting technology,dynamic sampling from experimental animals at multiple moments to observe the changes of dopamine in striatum with ischemia.And observe the effect of EA at Fengchi(GB 20)on such changes.Result: About the extracellular level of DA in normal group,sham-operation group and sham-operation + EA group,no significant changes were observed during the measuring period.The extracellular level of DA peaked at 15-45 min after MCAO and 0-30 min (P<0.05) after reperfusion.A rising trend was also observed following 120 min after reperfusion.After EA,the extracellular level of DA was significant lower than ischemia group following 90 min after reperfusion (P<0.05).And there was no peak again.Conclusion: EA at Fenchi(GB 20)can regulate the disorder of DA.EA can improve the integrity neurological function.This may be one of the mechanisms of acupuncture treating the lesion of cerebral ischemia and reperfusion.
Electroacupuncture Chromatography High Pressure Liquid Microdialysis Points Fengchi(GB 20) Dopamine Rats
XU Ming-shu GE Lin-bao XU Jia
Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.China Shanghai Research Center of Acupuncture and Meridian,Shanghai 201203,P.R.China;Shanghai Research Ins Shanghai Yue-yang Integrated Medicine Hospital Affiliated to Shanghai University of TCM,Shanghai 220
国际会议
The 3rd Workshop on Scientific Approaches to Chinese Medicine(第三届中医科学研究方法研讨会)
安徽黄山
英文
295-297
2008-10-25（万方平台首次上网日期，不代表论文的发表时间）