Effects of Laser Acupuncture on HSP70 Expression and Arthritic Histopathology in C57 Black Mice with Osteoarthritis
Methods: Sixty C57 black mice aged 10 weeks were divided randomly into six groups.Ten mice as normal control and fifty mice received forced running for 6 weeks as model of spontaneous osteoarthritis.In four treated groups,forty mice receiving either a 90-second treatment with a combined or single 10.6 μm laser or 650 nm laser irradiation or a sham treatment 3 times a week for 4 weeks on acupoint Dubi (ST 35)of left leg.All the mice were sacrificed and the left knee joints were removed at 20th week.Histopathologic changes and presence of heat shock protein (HSP) of arthritic cartilage were identified by hematoxylin and eosin stain and by immunostains of HSP 70 antibody individually from frozen sections of arthritic cartilage.Histological changes in cartilage structure were assessed and quantified with the modified Mankin scoring system.
ZHAO Ling SHEN Xue-yong CAO Yue-long DENG Hai-ping CHENG Ke ZHANG Hai-meng
Shanghai University of Traditional Chinese Medicine,Shanghai 201203,P.R.China Shanghai University of Traditional Chinese Medicine,Shanghai 201203,P.R.China;Shanghai Research Cent
国际会议
The 3rd Workshop on Scientific Approaches to Chinese Medicine(第三届中医科学研究方法研讨会)
安徽黄山
英文
288
2008-10-25（万方平台首次上网日期，不代表论文的发表时间）